Association of two complementary nucleic acid strands to form double-stranded molecules, which can contain two DNA strands, two RNA strands, or one DNA and one RNA strand.
: Any crossing of individuals of different genetic composition, often belonging to separate species, resulting in hybrid offspring.
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is the process of combining two complementary single-stranded DNA or RNA molecules and allowing them to form a single double-stranded molecule through base pairing.
In molecular biology, refers to the process by which sample DNA attached to a matrix (usually a membrane) is incubated with a labelled probe. The probe will anneal to the sample DNA where there is sufficient complementarity.
locating a gene by adding specific radioactive probes for the gene and detecting the location of the radioactivity on the chromosome after ...
In situ hybridization
(Science: molecular biology) The use of a dna or rna probe to detect the complementary dna sequence.
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bonding of complementary bases to form double-stranded molecules; this process is the basis for molecular biological techniques in which a labeled probe sequence is used to detect another identical or similar sequence (e.g., Southern , ...
A technique where a denatured (single-stranded) nucleotide chain (DNA or RNA) is allowed to pair with another single-stranded nucleotide chain.
is effective pollination between flowers of different species of the same genus, or even between flowers of different genera (as in the case of several orchids).
hybridization /HIGH-brid-eye-ZAY-shən, -brəd-/ n. (1) interbreeding between distinct genetic types, including types treated as distinct species; (2) the process of joining two complementary strands of DNA, or one each of DNA and RNA, ...
. The hydrogen bonding of complementary DNA and/or RNA sequences to form a duplex molecule. (See Northern , Southern .) ...
Hybridization: The specific reassociation of complementary strands of nucleic acids.
Hybrid vigor (heterosis): Unusual growth, strength, and health of heterozygote offspring from two less vigorous homozygote parents.
The process of joining two complementary strands of DNA or one each of DNA and RNA to form a double-stranded molecule.
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Hybridization with radioactive probe
A single locus probe is a DNA or RNA sequence that is able to hybridize (i.e. form a DNA-DNA or DNA-RNA duplex) with DNA from a specific restriction fragment on the Southern blot.
DNA The formation of hybrid DNA molecules that contain a strand of DNA from two different species. The number of complementary sequences in common in the two strands is an indication of the degree of relatedness of the species.
As we saw in the comparison of human and kangaroo cytochrome c, a single molecule provides only a narrow window for glimpsing evolutionary relationships.
In situ A technique for mapping cloned DNA sequences by directly to metaphase chromosomes and analysis by microscopy (see Chapter 10).
See In situ in the MGI Glossary.
When an atom forms covalent bonds, the s orbital and the p orbitals of the valence shell may become rearranged to form four new hybrid orbitals. The red structures in the model below represent the hybridized orbitals.
in situ - detection of the location of nucleic acid sequences in a cell or organism.
inducer gene - gene encoding the repressor protein of the lac operon; when lactose binds the repressor protein, the lac operon is induced.
In situ hybridization
For analysis of endogenous gene expression, wild-type and transgenic lungs at E18.5 were fixed in 4% paraformaldehyde/phosphate-buffered saline, dehydrated and embedded in paraffin, and 7 μm sections cut.
... with two alleles each ... Molecular Research Center develops solutions, including High Efficiency systems, Super hyb Kit, formazol, for Southern, northern, dot ...
Hybridization conditions: higher the stringency, lower the probability of hybridization. Increase the temperature or decreasing the salt concentration raises the stringency.
Hybridization Melting temperature ...
- Production of offspring, or hybrids, from genetically dissimilar parents. In selective breeding, it usually refers to the offspring of two different species.
Hybridization: The reaction by which the pairing of complementary strands of nucleic acid occurs. DNA is usually double-stranded, and when the strands are separated they will re-hybridize under the appropriate conditions.
A technique that enables researchers to determine the presence of certain nucleotide sequences in a sample of DNA.
DNA hybridization (exploiting the fundamental principle of complementary base pairing) studies have been used to reveal the relationships between species that could not be resolved by other means.
(iv) with radioactive probes
(v) Restriction fragment preparation
A: (ii) Blotting ...
In situ hybridization of a genome. This technique allows the discrimination between chromosomes originating from different species present within one nucleus.
Use of a DNA or RNA probe to detect the presence of the complementary DNA sequence in chromosome spreads or in interphase nuclei or an RNA sequence of cloned bacterial or cultured eukaryotic cells.
In-situ hybridization is a process that allows us to see which cells are expressing a particular mRNA. The way we do that is we make a probe that is similar to that mRNA, but it's an anti-sense probe.
Replicate The set of s that are performed with similar samples and arrays, which can be averaged. Replicates can be technical or biological.
Polyploidy and hybridization are important speciation mechanisms in plants. Whereas animals tend to be unisexual, plants often have both sexes functional in the same individual.
DNA -- a technique for selectively binding specific segments of single-stranded (ss) DNA or RNA by base pairing to complementary sequences on ssDNA molecules that are trapped on a nitrocellulose filter.
This spontaneous hybridization may lead to a 'superweed,' which may have a selective advantage and be difficult to control.
Some crops do hybridize with weedy relatives, and crop-to-weed transgene escape is a possibility.
DNA renaturation () - process whereby two complementary nucleic acid strands form a double helix during an annealing period; a powerful technique for detecting specific nucleotide sequences ...
Speciation through hybridization and/or polyploidy has long been considered much less important in animals than in plants [[[refs.]]]. A number of reviews suggest that this view may be mistaken.
Cross-. The hydrogen bonding of a single- stranded DNA sequence that is partially but not entirely complementary to a singlestranded substrate.
Smaller variants, particularly CNVs, segmental duplications, and interchromosomal interstitial rearrangements, are assessed by fluorescence in situ hybridization (FISH) or competitive genomic hybridization arrays (aCGH).
1859 Charles Darwin publishes The Origin of Species 1865 Gregor Mendel's paper, Experiments on Plant 1903 Chromosomes are discovered to be hereditary units 1905 British biologist William Bateson coins the term "genetics" in a letter to ...
See also: Hybrid, DNA, Trans, Organ, Sequence